Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rena Wang Yuan
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 09:46:18
Promoter+RBS+SacB+RBS+GlgC+Terminator
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1600004_sequence (Version 1) |
Description
This composite part was designed to be a sugar polymerization circuit in order to polymerize free simple sugars into their heavy-weight polymers intracellularly. The GlgC gene codes for a protein to polymerize glucose into glycogen. On the other hand, the SacB gene codes for a protein to polymerize fructose into levan and also confers a fructose sensitivity to the chassis. Both components of this part, GlgC and SacB, were studied separately in order to better characterize them. With GlgC alone in a BioBrick device, we found by spectrophotometric analysis that in 10% glucose solution, the E. coli exhibited a 1.5-fold increase in the glycogen production whereas the same BioBrick did not polymerize fructose, as expected in a control. In addition to the glucose polymerization, this bioBrick device demonstrated an ability to inhibit E. coli growth by up to 20% in 10% fructose solution, thereby showing at some evidence of conferred sensitivity due to the SacB gene.Notes
None.Source
Xl1 Blue E. coli K12 substrain.| Access | Instance | Definition |
| public public public public | BBa_J23110 BBa_K118018 BBa_K322921 BBa_B0015 | BBa_J23110 BBa_K118018 sacB BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23110 BBa_K118018 BBa_K322921 BBa_B0015 | 1,35 44,1358 1367,2803 2812,2940 | BBa_J23110 BBa_K118018 sacB BBa_B0015 |