Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Stukenberg
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-19 12:10:04
Composite carrying the Lac-inducible CDI-operon,constitutively expressed RFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1650009_sequence (Version 1) |
Description
Note: This part is similar to BBa_K1650007 except that it does not contain lacI and RFP expression is driven by a constitutive promoter instead of a lac-inducible promoter.The composite part contains a lac-inducible CDI operon which gives your strain the capability to inhibit growth of bacterial cells that express BamA in their outer membrane (most gram negative bacteria). Additionally the part contains mRFP1 (BBa_E1010) driven by constitutive promoter.
IPTG-induced cells harboring BBa_K1650009 express CdiB which forms a beta-barrel structure. The CdiA protein is transported through CdiB and is displayed on the cell surface. When getting in contact with BamA of a target cell, the C-terminal domain is delivered into the target cell acting as pore forming protein thus destroying the proton motoric force of the target cell. BBa_K1650009 could be used to improve transformation efficiency. Induced CDI+ cells would kill false positive cells
Notes
BBa_K1650009 was toxic for cells in pSB1C3 backbone, presumably because of the high metabolic burden. In accordance with iGEM Headquarter, we submitted BBa_K1650009 in the low copy pSB4C5 backboneSource
The CDI-operon was PCR amplified from genomic DNA of E.coli strain EC93. Other parts were taken from the registry.| Access | Instance | Definition |
| public public public public public public public public public | BBa_K823008 BBa_B0034 BBa_E1010 BBa_B0010 BBa_K1650033 BBa_K1650034 BBa_K1650013 BBa_B0053 BBa_B0054 | BBa_K823008 BBa_B0034 mRFP1 BBa_B0010 BBa_K1650033 BBa_K1650034 CDI BBa_B0053 BBa_B0054 |