Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brian Dempsey
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 02:28:53
Nuclease - secreted with Cterm 6xHis tag
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1792004_sequence (Version 1) |
Description
Nuclease from Staphylococcus aureus. This nuclease is secreted due to a bacterial N-terminal export tag. The nuclease contains a C-terminal 6xHis tag for possible purification and concentration. This part is the same as BBa_K729004 with the addition of the his-tag. Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides (Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.)The protein is undercontrol of an IPTG inducible promoter.
Notes
The first 64 amino acids have been removed (not necessary for function). It has a functional bacterial N-terminal secretion tag that is 19 amino acids in length.Source
This is a genomic sequence of Nuclease from Staphylococcus aureus. Found in the UniprotKB database: P00644 (NUC_STAAU). The first 64 amino acids have been removed (not necessary for function). It has a functional bacterial N-terminal secretion tag that is 19 amino acids in length.| Access | Instance | Definition |
| public public public | BBa_K613010 BBa_K1792005 BBa_B0015 | BBa_K613010 BBa_K1792005 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K613010 BBa_K1792005 BBa_B0015 | 1,114 121,696 705,833 | BBa_K613010 BBa_K1792005 BBa_B0015 |