BBa_K1792004

BBa_K1792004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1792004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brian Dempsey
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 02:28:53

Nuclease - secreted with Cterm 6xHis tag



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1792004_sequence (Version 1)

Description

Nuclease from Staphylococcus aureus. This nuclease is secreted due to a bacterial N-terminal export tag. The nuclease contains a C-terminal 6xHis tag for possible purification and concentration. This part is the same as BBa_K729004 with the addition of the his-tag. Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides (Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.)

The protein is undercontrol of an IPTG inducible promoter.

Notes

The first 64 amino acids have been removed (not necessary for function). It has a functional bacterial N-terminal secretion tag that is 19 amino acids in length.

Source

This is a genomic sequence of Nuclease from Staphylococcus aureus. Found in the UniprotKB database: P00644 (NUC_STAAU). The first 64 amino acids have been removed (not necessary for function). It has a functional bacterial N-terminal secretion tag that is 19 amino acids in length.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1792004/1