Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samuel Magaziner
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 04:06:50
LuxR/AHL driven Lysis Cassette with Protein level Timer Delay
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1848006_sequence (Version 1) |
Description
This system presents an AHL/LuxR dependent mechanism for delayed cell lysis. Following induction with AHL, the repressible pLux becomes inhibited and ceases producing repressor protein cI. Following this the pcI becomes uninhibited and proceeds to translate regions encoding lysis inducing proteins. Taken together with the need for AHL/LuxR complexation and cI's ceased production, this exemplifies a time delayed quorum sensing dependent kill switch for the cell. Note: this part does not include the LuxR coding region. Without this within the plasmid as well, the system will not function.Notes
We had to balance potential basal levels of lysis expression. Luckily, this proved not to be an issue.Source
The quorum sensing components come from Vibrio fischeri. The cI components hail from the T4 enterobacteria phage.| Access | Instance | Definition |
| public public public public public | BBa_R0061 BBa_B0034 BBa_C0051 BBa_R0051 BBa_K112808 | luxR BBa_B0034 cI lam cI lam BBa_K112808 |