Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lejian Jiang
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-06 05:47:06
AND GATE-input
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1886007_sequence (Version 1) |
Description
This is the input part of AND GATE. After being induced by IPTG, plac promoter activates the expression of T7 RNA polymerase which contains two amber mutations (T7 ptag) . After being induced by L-arabinose, pBAD promoter activates the expression of a special tRNA (supD), which is used to identify amber mutation. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase. Therefore, only when the two inputs exist together, T7 RNA polymerase can be expressed, and then T7 promoter can be activated to express the downstream gene.Notes
PT7(BBa_K1321338) contains T7 promoter and RBS(B0034, which enables easy addition to any coding part which does not already have these parts.Source
This part is obtained from iGEM DNA Distribution kits, created by iGEM PKU Team in 2009.| Access | Instance | Definition |
| public public public public public public public | BBa_I13453 BBa_K228001 BBa_B0015 BBa_R0010 BBa_B0033 BBa_K228000 BBa_B0015 | BBa_I13453 BBa_K228001 BBa_B0015 LacI BBa_B0033 BBa_K228000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13453 BBa_K228001 BBa_B0015 BBa_R0010 BBa_B0033 BBa_K228000 BBa_B0015 | 1,130 139,274 283,411 420,619 628,638 645,3317 3326,3454 | BBa_I13453 BBa_K228001 BBa_B0015 LacI BBa_B0033 BBa_K228000 BBa_B0015 |