BBa_K1886013

BBa_K1886013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1886013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lejian Jiang
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-19 11:12:23

AND GATE-input_DPD+ red light



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K1886013_sequence (Version 1)

Description

The circuit to receive input signals is composed of AND GATE, quorum sensing promoter and light-controlled promoter. After being induced by DPD, plsrA promoter activates the expression of T7 RNA polymerase which contains two amber mutations(T7ptag) . The expression of a special tRNA (supD), which is used to identify amber mutation, is activated by Pλ promoter without the restraint of CL protein. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase, and red light could inhibit the produce of CL protein(for more information, see the part"PompC-NOT Gate"). Therefore, when DPD and green light exist together, T7 RNA polymerase will be expressed, and then T7 promoter will be activated to express the downstream gene.

Notes

PT7(BBa_K1321338) contains T7 promoter and RBS(B0034, which enables easy addition to any coding part which does not already have these parts.

Source

This part is a combination of DPD input and Red light input. The subparts of this part are from iGEM Distribution Kits.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1886013/1