| Types | DnaRegion
|
| Roles | Composite
engineered_region
|
| Sequences | BBa_K1911000_sequence (Version 1)
|
Description
This part is a part of a larger construct which allows the system to change from one state to another. So when the E. coli cells containing the larger construct is induced with IPTG, the IPTG binds with the LacI repressor, allowing the other pathway to be expressed and it expresses a CI repressor which shuts down the 1st pathway. This part was created in order for teams to be able to easily place their genes of interest in this "switch" system. They would combine their gene of interest with the pLamdaR-LacI part and then proceed to combine it to the rest of the system.
Notes
We had to make sure there were no illegal restriction enzyme sites (EcoRI, PstI, SpeI, and XbaI) which cut the part in places where we did not want them to.
Source
The part was created by combining multiple parts found on the iGEM parts registry.
