BBa_K1913031

BBa_K1913031 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1913031
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianhe Wang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 04:06:49

Synthetic ptet-FixK2 hybrid promoter+RBS with mRFP



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1913031_sequence (Version 1)

Description

Synthetic ptet-FixK2 hybrid promoter composite part includes mRFP reporter (BBa_E1010). After co-transformation with light sensor BBa_K1913033, mRFP fluorescence could be detected after 12h culture.

Notes

In order to test synthetic ptet-FixK2 hybrid promoter,we put reporter mRFP under this promoter and co-transformed with light sensor BBa_K1913034. And mRFP fluorescence value was tested so that the intensity of transcription of this promoter could be quantified.

Source

Genomic sequence of Bradyrhizobium japonicum, iGEM part registry.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1913031/1