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BBa_K195619

BBa_K195619 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K195619

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yun-Hsiang Chang

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:17

pLacI with activator gene CII and pRE with GFP generator

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K195619_sequence (Version 1)

Description

This part is composed of pLac with inducer gene CII, and it would induce pRE to produce green fluorescent protein. The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from λ phage. The pLac is repressed by LacI, which could be inhibited by IPTG.The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pLac and produce green fluorescent protein.

Notes

none

Source

biobrick

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0010 BBa_B0034 BBa_K116602 BBa_B0010 BBa_B0012 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	LacI BBa_B0034 CII BBa_B0010 BBa_B0012 pRE BBa_B0034 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010 BBa_B0034 BBa_K116602 BBa_B0010 BBa_B0012 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	1,200 209,220 227,520 529,608 617,657 666,713 722,733 740,1459 1468,1547 1556,1596	LacI BBa_B0034 CII BBa_B0010 BBa_B0012 pRE BBa_B0034 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K195619/1