BBa_K1970002

BBa_K1970002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1970002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eivind B??e Drejer

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-27 07:17:50

Red fluorescent protein controlled by CI-repressor

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1970002_sequence (Version 1)

Description

This part is made up of a gene for red fluorescent protein (RFP) controlled by the lambda promoter. A CI-repressor gene is attached to the parts, which represses expression of RFP by binding to the lambda promoter.

Notes

The most challenging part was choosing the right ribosomal binding site and transcription terminator for the desired expression strength of the red fluorescent protein.

Source

Constructed from iGEM biobricks BBa_R0051, BBa_J06702 and synthesized CI protein gene, ribosomal binding site and transcription terminator.

Components

Access	Instance	Definition
public	BBa_B0034	BBa_B0034
public	BBa_B0012	BBa_B0012
public	BBa_R0051	cI lam
public	BBa_K608351	BBa_K608351
public	BBa_R0051	cI lam
public	BBa_B0034	BBa_B0034
public	BBa_E1010	mRFP1
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	44,55	BBa_B0034
BBa_B0012	797,837	BBa_B0012
BBa_R0051	900,948	cI lam
BBa_K608351	1,948	BBa_K608351
BBa_R0051	957,1005	cI lam
BBa_B0034	1014,1025	BBa_B0034
BBa_E1010	1032,1737	mRFP1
BBa_B0015	1746,1874	BBa_B0015

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1970002/1

Member of these Collections

• iGEM Parts Registry