Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shauna Winchester
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-17 01:47:37
Mg-Chelatase Plasmid
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1998000_sequence (Version 1) |
Description
This composite part is made up of various genes that form a part of the chlorophyll biosynthesis pathway. ChlI1 and ChlI2 forms a Mg-chelatase complex which catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. ChID forms an ATP dependent complex with ChlI1 and ChlI2 before acting of protoporphyrin. GUN4 is a tetrapyrrole-binding protein which controls the production of Mg-protoporphyrin IX found only in aerobic photsynthetic organisms. It is thought to participate in plastid-to-nucleus signalling by regulating Mg-protoporphyrin IX synthesis. Finally, ChlH is a chloroplast precursor which plays a role in the regulation of light in chlorophyll biosynthesis.CTH1 is a functional protein that is developed in the prescense of oxygen or copper. It catalyzes Mg-protoporphyrin IX monomethyl, which converts to divinyl protochlorophyllide in the presence of NADPH and O2.
Notes
Codon optimisation, order of parts in operon, illegal enzyme kinetics.Source
The genes were sourced from Chlamydomonas rienhardtii and synthesised.| Access | Instance | Definition |
| public public public public public | BBa_K1326008 BBa_K1080011 BBa_K1080005 BBa_R0010 BBa_K1640019 | BBa_K1326008 "Eye 2" "CTH1" LacI BBa_K1640019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1326008 BBa_K1080011 BBa_K1080005 BBa_R0010 BBa_K1640019 | 1,4222 4231,5442 5451,6602 6611,6810 6819,10938 | BBa_K1326008 "Eye 2" "CTH1" LacI BBa_K1640019 |