Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Muhammad Ismail
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 01:10:17
NOx(Oxides of Nitrogen) sensor device (pNirK-Yellow chromoprotein)
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_K2056004_sequence (Version 1) |
Description
This part is the oxides of nitrogen (NOx) sensing module of the 'Inspector NOxCO' device. 'Inspector NOxCO' is a bacterial sensor for carbon monoxide (CO) and oxides of nitrogen (NOx) in vehicle emissions.This part consists of the Anderson constitutive promoter(BBa_J23110) and RBS(B0030) with the coding sequence for NsrR(K2056001) a NOx sensitive repressor protein and single terminator(B0010) followed by NsrR repressed promoter pNirk(k2056002) with RBS(B0030) and 'Yellow chromoprotein'(K1033910) with terminator(BBa_B0012).
If NOx are present, they will bind to the constitutive expressed NsrR that will relieve repression on the pNirK promoter activating transcription of the yellow chromoprotein.
Notes
The selection of the RBS and Constitutive promoter. Because of limitations of repeats that could be synthesized, we had to give up on the double terminators in the original design and go with single different terminators. A 70bp spacer(B0040) has been added for transcriptional efficiency.Source
All parts in this sequence come from the Registry and have been listed. However, the complete part has been synthesized from IDT using their free gBLocks offer.| Access | Instance | Definition |
| public public public public public public public public public | BBa_J23110 BBa_B0030 BBa_K2056001 BBa_B0010 BBa_B0040 BBa_K2056002 BBa_B0030 BBa_K1033910 BBa_B0012 | BBa_J23110 BBa_B0030 BBa_K2056001 BBa_B0010 spacer BBa_K2056002 BBa_B0030 fwYellow BBa_B0012 |