BBa_K2080010

BBa_K2080010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2080010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Haojian Li

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 05:39:44

bcl2-sgRNA1234

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K2080010_sequence (Version 1)

Description

This is a group of guide RNAs that have a hundred base-long molecule with a dCas9-KRAB scaffold sequence, which can guide dCas9-KRAB to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bcl-2, a anti-apoptosis protein, which can inhibit the transcription of bcl-2.

Notes

We design the sequence of the gRNA that is complementary to the bcl-2 promoter region, and try to get closer to the transcription start site to obtain higher repression of bcl-2 trascription.

Source

From
BBa_K2080004
BBa_K2080005
BBa_K2080006
BBa_K2080007

Components

Access	Instance	Definition
public	BBa_K2080004	BBa_K2080004
public	BBa_K2080005	BBa_K2080005
public	BBa_K2080006	BBa_K2080006
public	BBa_K2080007	BBa_K2080007

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2080004	1,367	BBa_K2080004
BBa_K2080005	376,742	BBa_K2080005
BBa_K2080006	751,1117	BBa_K2080006
BBa_K2080007	1126,1492	BBa_K2080007

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2080010/1

Member of these Collections

• iGEM Parts Registry