Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haoran Ding
Date created: 2016-10-12 11:00:00
Date modified: 2016-11-16 07:21:34
PETase expression system in Saccharomyces cerevisiae
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2110200_sequence (Version 1) |
Description
Saccharomyces cerevisiae is a widely used chassis organism in synthetic biology research. We use it as the chassis organism to express the PETase gene and assay the enzyme activity. This composite part consists of the enhanced promoter PGK1 (BBa_K2110012), α signal peptide (BBa_K2110017), PETase gene (or its various mutants, BBa_K2110800, BBa_K2110100-BBa_K2110109), and CYC1 terminator (BBa_K2110001). The expression data can be accessed respectively in the part information page of PETase gene and its mutants. (BBa_K2110800, BBa_K2110100-BBa_K2110109).Notes
It should be used in Saccharomyces cerevisiae.Source
BBa_K2110012, BBa_K2110017, BBa_K2110800 or BBa_K2110100-BBa_K2110109, BBa_K2110001| Access | Instance | Definition |
| public public public public | BBa_K2110012 BBa_K2110017 BBa_K2110800 BBa_K2110000 | BBa_K2110012 BBa_K2110017 BBa_K2110800 BBa_K2110000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2110012 BBa_K2110017 BBa_K2110800 BBa_K2110000 | 1,781 790,1056 1063,1857 1866,2113 | BBa_K2110012 BBa_K2110017 BBa_K2110800 BBa_K2110000 |