Types | DnaRegion
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Roles | engineered_region
Measurement
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Sequences | BBa_K2115000_sequence (Version 1)
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Description
This composite is made by LacI promoter, which is inducible by IPTG molecule; After LacI promoter is activated,it will also activate the transcription of CooA, a transcription factor that can detect carbon monoxide.
LacI promoter is an indispensble part, since without it the bacteria would be in an unnecessary operation.
Notes
The only consideration was the bioinformatic assembly this parts, because we had to learn to use Artemis software.
Source
LacI: genomically it comes from E.coli bacterium, however we took this promoter from the IGEM catalog.
RBS: ribosome binding site.
CooA: a transcription factor present in the Rhodospirillum Rubrum protobacteria.
Terminators: an RNA sequence that usually occurs at the end of a gene or operon mRNA and causes transcription to stop.