View

BBa_K2187000

BBa_K2187000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2187000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ella Coleman

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 04:23:01

Lipase gene

Back

Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K2187000_sequence (Version 1)

Description

The Lipase gene comes from the Ideonella sakaiensis bacteria. Its purpose is to create and enzyme called MHETase that breaks down MHET into ethylene glycol and terephthelic acid.

Notes

We added a lacl regulated promoter, BBa_R0011, so that we could induce it to very high levels of expression. We also added a prefix and suffix, a BBa_B0030 RBS, and a BBa_B0015 terminator.

Source

We used the sequence of the genomic DNA:GAP38373.1 from GenBank.

Components

Access	Instance	Definition
public public public public	BBa_R0011 BBa_K2187002 BBa_B0030 BBa_B0015	lacI+pL BBa_K2187002 BBa_B0030 BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0011 BBa_K2187002 BBa_B0030 BBa_B0015	1,54 62,937 946,960 969,1097	lacI+pL BBa_K2187002 BBa_B0030 BBa_B0015

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Planning
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K2187000/1