BBa_K228109

BBa_K228109 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K228109

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shan Shen

Date created: 2009-09-18 11:00:00

Date modified: 2015-05-08 01:11:32

LacP(R0010) + RBS(B0031) + T7ptag(K228000) + terminator(B0015)

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K228109_sequence (Version 1)

Description

T7 polymerase with an amber mutation coding gene is constructed after Lac promoter which would be triggered by IPTG. T7 polymerase can be well expressed in the exsitence of SupD tRNA. Also, the expression of the T7 polymerase can be regulated by RBS. It is one of the LacP-rbs-T7ptag-terminator family members, ditinguished by different rbs.

Source

It is made up of standard subparts from the partregistry.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0031	BBa_B0031
public	BBa_K228000	BBa_K228000
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0031	209,222	BBa_B0031
BBa_K228000	229,2901	BBa_K228000
BBa_B0010	2910,2989	BBa_B0010
BBa_B0012	2998,3038	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K228109/1

Member of these Collections

• iGEM Parts Registry