Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan Shen
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:32
AND GATE AraC + SupD + LacP +RBS(B0033)+T7ptag
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K228113_sequence (Version 1) |
Description
This Device belongs to the SupD_T7ptag AND Gate family constructed by Peking University 2009 iGEM TeamThis is a AND GATE in the Ecoli stain JM109. Use arabinose to induce the transcription of SupD and use IPTG to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.
Note: this AND gate make use of the lacI on the F plasmid of JM109, and because of the instability of the F plasmid, the leakage of lacP is significant. For improvement, a lacI generator can be assemble into it. This gate only works on low copy plasmid, such as pSB4K5, due to the lacI on the F plasmid.
This gate is only one of nine gates(K228110-K228118), which are different in the RBS of T7ptag.
For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
Source
It is made up of standard subparts from the partregistry.| Access | Instance | Definition |
| public public public public public | BBa_B0010 BBa_B0012 BBa_K228009 BBa_K228100 BBa_K228108 | BBa_B0010 BBa_B0012 BBa_K228009 BBa_K228100 BBa_K228108 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0010 BBa_B0012 BBa_K228009 BBa_K228100 BBa_K228108 | 1013,1092 1101,1141 1,1279 1288,1560 1569,4603 | BBa_B0010 BBa_B0012 BBa_K228009 BBa_K228100 BBa_K228108 |