Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marc T. K. Nielsen
Date created: 2009-08-02 11:00:00
Date modified: 2015-05-08 01:11:36
IPTG inducible sRIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K237006_sequence (Version 1) |
Description
RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species. RIP effectively works as an antibiotic in its inhibition of bacterial growth.RIP has the sequence YSPXTNF and has been shown through extensive research to inhibit bacterial infections in mice in a synthetic (as in organic synthesis) form.
The RIP produced by this generator is an export-tagged RIP brick. We used an OmpA tag, which (at least in theory) targets the peptide to the periplasmic space from where, there has been described numerous cases of leakage into the medium. Under the transport into the periplasmic space, the OmpA-leader should be cut off, leaving the RIP intact, and ready to inhibit some staph's.
References will come later.
Notes
There are 4 versions of RIP producers from us.1 - Constitutively produced RIP without an export sequence
2 - Inducibly produced RIP without an export sequence
3 - Constitutively produced RIP with an export sequence (OmpA)
4 - Inducibly produced RIP with an export sequence (OmpA)
This is number 4