Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-13 11:00:00
Date modified: 2015-05-08 01:11:37
Strep-DigA-Split Linker-Fok_a
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K243017_sequence (Version 1) |
Description
These part units some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain foka, make it possible to purifier and detect the function of the protein in one step. The fluorescineA tag allows the measurement by quenching and the coupling to a anticalin linked oligo.Notes
The choice of the linker, lipocalin tag and purification tag allows us several different combinations.Source
Combined the parts by serial cloning steps.| Access | Instance | Definition |
| public public public public public public public | BBa_K157012 BBa_B0105 BBa_K243003 BBa_B0105 BBa_K157009 BBa_B0105 BBa_K243000 | BBa_K157012 Scar 25 DigA Scar 25 linker Scar 25 Fok_a |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K157012 BBa_B0105 BBa_K243003 BBa_B0105 BBa_K157009 BBa_B0105 BBa_K243000 | 1,24 25,30 31,582 583,588 589,639 640,645 646,1224 | BBa_K157012 Scar 25 DigA Scar 25 linker Scar 25 Fok_a |