BBa_K243017

BBa_K243017 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K243017
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-13 11:00:00
Date modified: 2015-05-08 01:11:37

Strep-DigA-Split Linker-Fok_a



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K243017_sequence (Version 1)

Description

These part units some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain foka, make it possible to purifier and detect the function of the protein in one step. The fluorescineA tag allows the measurement by quenching and the coupling to a anticalin linked oligo.

Notes

The choice of the linker, lipocalin tag and purification tag allows us several different combinations.

Source

Combined the parts by serial cloning steps.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K243017/1