BBa_K243022

BBa_K243022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K243022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Freiburg Bioware09

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:37

DsbA-Strep-DigA-Split Linker-Fok_a

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K243022_sequence (Version 1)

Description

The DsbA tag is used to transfer the produced protein into the periplasm. This makes is easier for us to extract the protein.

Notes

none

Source

Combined by serial cloning steps

Components

Access	Instance	Definition
public	BBa_K243002	DsbA
public	BBa_B0105	Scar 25
public	BBa_K157012	BBa_K157012
public	BBa_B0105	Scar 25
public	BBa_K243003	DigA
public	BBa_B0105	Scar 25
public	BBa_K157009	linker
public	BBa_K243000	Fok_a

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K243002	1,54	DsbA
BBa_B0105	55,60	Scar 25
BBa_K157012	61,84	BBa_K157012
BBa_B0105	85,90	Scar 25
BBa_K243003	91,642	DigA
BBa_B0105	643,648	Scar 25
BBa_K157009	649,699	linker
BBa_K243000	700,1278	Fok_a

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K243022/1

Member of these Collections

• iGEM Parts Registry