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BBa_K318014

BBa_K318014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K318014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nathaniel Pantalone, Justin Vrana

Date created: 2010-07-12 11:00:00

Date modified: 2015-05-08 01:11:57

pCons + attB + RBS + GFP + T + rRFP + rRBS + attP

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Details

Types	DnaRegion
Roles	engineered_region Device
Sequences	BBa_K318014_sequence (Version 1)

Description

This composite part is a testing cassette for a Cre-like, P22 Int recombination. The constitutive promoter should express GFP if recombination does not occur. If Cre-like recombination occurs, this part should express RFP. It is composed of the following biobricks: BBa_J23100, BBa_I11032, BBa_E0240, BBa_K199021, BBa_I11033.

Notes

The length of DNA between attB and attP might not be sufficient for recombination.

Source

Biobricks

Components

Access	Instance	Definition
public public public public public public public public public	BBa_J23100 BBa_I11032 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012 BBa_J31008 BBa_J44001 BBa_I11033	BBa_J23100 P22 attB BBa_B0032 GFP BBa_B0010 BBa_B0012 mRFPr BBa_J44001 P22 attP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23100 BBa_I11032 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012 BBa_J31008 BBa_J44001 BBa_I11033	1,35 44,70 79,91 98,817 826,905 914,954 963,1640 1649,1663 1672,1931	BBa_J23100 P22 attB BBa_B0032 GFP BBa_B0010 BBa_B0012 mRFPr BBa_J44001 P22 attP

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K318014/1