Types | DnaRegion
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Roles | Device
engineered_region
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Sequences | BBa_K331000_sequence (Version 1)
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Description
This part is one that when induced by IPTG will form a microcompartment made of Lumazine Synthase. This will the arginine tails of parts BBa_K249004 and BBa_K294005 will allow for the targeting of your protein of interest into the microcompatment.
Notes
We followed Seebeck et al. and mutated Arg83, Thr 86, Thr 120, and Gln123 to glutamates
Source
Seebeck, F. P., Woycechowsky, K. J., Zhuang, W., Rabe, J. P., and Hilvert, D., (2006). A simple tagging system for protein encapulation. J. Am. Chem. Soc. 128, 4516-4517