Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonas Aretz
Date created: 2010-10-10 11:00:00
Date modified: 2015-05-08 01:12:19
vir promoter + sensitivity tuner + luciferase
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K389413_sequence (Version 1) |
Description
This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.Notes
sensitivity tuner to enhance the luciferase signalSource
- luciferase from pGL4.10luc2 (Promega)
- sensitivity tuner part, terminators and constitutive promoter from the partsregistry
- virG was synthesized by Mr. Gene
- vir promoter from Agrobacterium tumefaciens C58, TI-plasmid
| Access | Instance | Definition |
| public public public public public | BBa_J23110 BBa_K389002 BBa_B0017 BBa_K389003 BBa_K389403 | BBa_J23110 virG BBa_B0017 Pvir BBa_K389403 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23110 BBa_K389002 BBa_B0017 BBa_K389003 BBa_K389403 | 1,35 44,783 792,959 968,1125 1134,4047 | BBa_J23110 virG BBa_B0017 Pvir BBa_K389403 |