BBa_K412000

BBa_K412000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K412000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kristina Ehrhardt
Date created: 2010-10-14 11:00:00
Date modified: 2015-05-08 01:12:26

PyeaR+GFP



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K412000_sequence (Version 1)

    Description

    PyeaR+GFP is a nitrate/nitrite detector. PyeaR (BBa_K216005) is a nitrate/nitrite inducible promoter, that we ligated to GFP (I13504), so that we could detect nitrates and nitrites.

    Notes

    We knew we wanted a nitrate sensitive promoter followed by a reporter. Therefore we digested PyeaR with SpeI and PstI and digested GFP with XbaI and PstI and then ligated the two parts together to form this composite part.

    Source

    We got both subparts from the registry, when we looked for a nitrate detector and a reporter.

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K412000/1
     

    Attachments

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