Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero, Felix Reyes
Date created: 2011-10-21 11:00:00
Date modified: 2015-05-08 01:12:30
pUC18Sfi-miniTn7BB-Gm-improved_flipflop_(module I)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K510045_sequence (Version 1) |
Description
This plasmid allows the integration of the improved flip-flop (module I) (part: BBa_K510019) into bacterial chromosomes using the pUC18Sfi-miniTn7BB-Gm (BBa_K51000) characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.For more information about the function of the miniTn7 BioBrick toolkit, visit the iGEM team UPO-Sevilla 2011 wiki.
Notes
The BBa_K510019 BioBrick was inserted within the BCS of pUC18Sfi-miniTn7BB-Gm (BBa K510000) by EcoRI and PstI clonning. In order to construct the pUC18Sfi-miniTn7BB-Gm delivery plasmid, the miniTn7BB-Gm transposon was cleaved from the commercial plasmid pMA (Mr. Gene) with SfiI and ligated to SfiI-digested pUC18Sfi. This strategy removes all multi-cloning restriction sites from pUC18Sfi, except for the flanking SfiI, thus guaranteeing that the unique sites in the transposon are not duplicated. The remove of the multi-cloning restriction sites was verified by analytic digestions.Source
The miniTn7BB-Gm minitransposon was synthesized commercially and pUC18SfiI is a commercial vector.The improved flip-flop (module I) was synthesized commercially.
| Access | Instance | Definition |
| public public | BBa_K510000 BBa_K510019 | BBa_K510000 BBa_K510019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510000 BBa_K510019 | 1,4388 4397,9074 | BBa_K510000 BBa_K510019 |