Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero, Felix Reyes
Date created: 2011-10-21 11:00:00
Date modified: 2015-05-08 01:12:30
pUC18R6KT-miniTn7BB-Gm-improved_flipflop_(module I)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K510046_sequence (Version 1) |
Description
This plasmid allows the integration of the improved flip-flop (module I) (part: BBa_K51019) into bacterial chromosomes using the pUC18Sfi-miniTn7BB-Gm (BBa_K51012) characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.For more information about the function of the miniTn7 BioBrick toolkit and flip-flops, visit the iGEM team UPO-Sevilla 2011 wiki.
Notes
The BBa_K510019 BioBrick was inserted within the BCS of pUC18R6KT-miniTn7BB-Gm (BBa K510012) by EcoRI and PstI clonning.Source
The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg.The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.
The improved flip-flop (module I) was synthesized commercially too.
| Access | Instance | Definition |
| public public | BBa_K510012 BBa_K510019 | BBa_K510012 BBa_K510019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510012 BBa_K510019 | 1,4549 4558,9235 | BBa_K510012 BBa_K510019 |