Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yang, Yi-Pei
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:37
crtEBI+RNA thermometer+crtY+terminator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K539142_sequence (Version 1) |
Description
CrtEBI with RBS that is assembled by previous team is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs) and Part BBa_K118005 (CrtI with rbs), which are submitted by previous iGEM teams. Together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). And then crtY(lycopene B-cyclase) converts lycopene to orange beta-carotene. The RNA thermometer that can be used for temperature sensitive post-transcriptional regulation which is based on a RNA thermometer from Salmonella Enterica Tyhpy initiates translation at 37??C. Therefore crtY expresses significantly at 37??C. The native terminator is included.Notes
We design this composite biobrick to show red and orange pigment at different temperature. Lower than 37??C the pigment is red lycopene and at 37??C the pigment is orange.Source
CrtEBI gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This composite biobrick come from BBa_K274100.CrtY comes from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321).
RNA thermometer comes from Salmonella Enterica Tyhpy (NC_010102)).
| Access | Instance | Definition |
| public public | BBa_K274100 BBa_K539138 | BBa_K274100 BBa_K539138 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K274100 BBa_K539138 | 1,3385 3394,4724 | BBa_K274100 BBa_K539138 |