Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Rodriguez
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:43
E. coli MxRed
| Types | DnaRegion |
| Roles | chromosome Cell |
| Sequences | BBa_K566019_sequence (Version 1) |
Description
Derived from Dr. Jeff Tabor JT2 E. coli strain, this part is a built-in red-light induction system in E. coli made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher.It is proposed as a photo-chassis that could make useful tools in this field of light induction.
Integration was performed for the genes pcyA, ho1, Cph8 and a Mnt working as follows:
Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor. These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, which stops being induced in presence of red-light. It is therefore used as a NOT-gate to regulate expression from pMnt.
Notes
Integration performed through protocol of Dr. KuhlmannSource
E. coli JT2 strain| Access | Instance | Definition |
| public public public public | BBa_K566026 BBa_K566020 BBa_K566023 BBa_K566024 | Cph8 gene BBa_K566020 BBa_K566023 BBa_K566024 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K566026 BBa_K566020 BBa_K566023 BBa_K566024 | 1,2436 2445,3028 3037,3984 3993,4916 | Cph8 gene BBa_K566020 BBa_K566023 BBa_K566024 |