BBa_K592027

BBa_K592027 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K592027
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lei Sun
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:49

T5-lac promoter-B0032-BFP



    • Details

    Types
    DnaRegion

    Roles
    Measurement

    engineered_region

    Sequences BBa_K592027_sequence (Version 1)

    Description

    The promoter T5-lac (BBa_K592008) for testing its promoter strength coupled with BFP (BBa_K592100). This construct should be cloned into pSB3K3 plasmid to measure the BFP output and thereby determine the promoter strength. Theoretically, the promoter should be well-repressed in LacIq strain but inducible by high concentrations of IPTG.

    Notes

    This part was assembled into pSB3K3 for testing, and later cloned into pSB1C3 for submission.

    Source

    The information of BBa_K592008 and BBa_K592100 can be found in the registry. T5-lac promoter is a fusion promoter with parts from T5 phage.

    Access Instance Definition
    public
    public
    public
    		BBa_K592008
    BBa_B0032
    BBa_K592100
    		PT5lac
    BBa_B0032
    BFP

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K592027/1
     

    Attachments

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