Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Philipp B??ing
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:13:04
Gyrase cassette
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K676016_sequence (Version 1) |
Description
DNA gyrase is the only topoisomerase that introduces negative supercoiling into closed circular plasmid molecules. It is a heterotetramer consists of two GyrA and two GyrB subunits.This expression cassette is regulated by an IPTG inducible promoter and will be expressing both Gyr A and Gyr B subunits . Gyr A subunit will bind to the DNA ( gyrase binding site) and is responsible for catalysing DNA breakage and resealing in order to change the level of supercoiling in DNA. Gyr B subunit provides energy for the reaction in the presence of divalent cations such as magnesium cation.
Notes
GyrB has a EcoR1 restriction site at 6th bp of the ORF, and it has been removed site directed mutagenesis.The wild type Gyr A ORF has a Pst 1 restriction site at the 1212th bp position so site directed mutagenesis was carried out to remove the restriction site.
Source
E.coli genome| Access | Instance | Definition |
| public public public public public | BBa_R0010 BBa_B0034 BBa_K676007 BBa_K676009 BBa_B0015 | LacI BBa_B0034 BBa_K676007 BBa_K676009 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_B0034 BBa_K676007 BBa_K676009 BBa_B0015 | 1,200 209,220 227,2854 2861,5275 5284,5412 | LacI BBa_B0034 BBa_K676007 BBa_K676009 BBa_B0015 |