Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjie Wu and Minghao Gong
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:08
GalK_Comparator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K737047_sequence (Version 1) |
Description
This device mean to test feasibility of N/P NOR GATE,replacing Nitrate,Phosphate inputs by IPTG,aTc. Spot42 sRNA is under the control of aTc inducible device,another RNA antiGalK is under the control of IPTG inducible device.AntiGalK stands for the RNA transcripted fully complementary to galK,shows modest repression on galK::GFP mRNA.
Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK???s RBS is a moderate one.
Notes
[1]Chase L. Beisel and Gisela Storz, The Base-Pairing RNA Spot 42 Participates in a Multioutput Feedforward Loop to Help Enact Catabolite Repression in Escherichia coli. Molecular Cell 41, 286???297, February 4, 2011Source
No| Access | Instance | Definition |
| public public public public public public public public public public public | BBa_J23106 BBa_P0440 BBa_R0011 BBa_K737033 BBa_J23106 BBa_P0412 BBa_R0040 BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 | BBa_J23106 BBa_P0440 lacI+pL BBa_K737033 BBa_J23106 BBa_P0412 p(tetR) BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 |