psb1C3_Int

BBa_K743005 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K743005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:09

psb1C3_IntK plasmid with RFP under psbA2 promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K743005_sequence (Version 1)

Description

This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome.
The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly.
It was used by UC_Chile 2012 team to succesfully transform Synechocystis http://2012.igem.org/Team:UC_Chile

Source

RS1 and RS2 were obtained from Synechocystis genomic DNA, both the kanamycin resistance and the double terminator are from the registry of standard biological parts.

Components

Access	Instance	Definition
public	BBa_K743000	RS1
public	BBa_K390017	BBa_K390017
public	BBa_E1010	mRFP1
public	BBa_B0015	BBa_B0015
public	BBa_K292003	BBa_K292003
public	BBa_K743001	RS2

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K743000	1,522	RS1
BBa_K390017	531,617	BBa_K390017
BBa_E1010	624,1304	mRFP1
BBa_B0015	1338,1466	BBa_B0015
BBa_K292003	1475,2544	BBa_K292003
BBa_K743001	2553,3239	RS2

Other Properties

• igem#experience: Works
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K743005/1

Member of these Collections

• iGEM Parts Registry