Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ilya Vainberg Slutskin
Date created: 2012-09-14 11:00:00
Date modified: 2015-05-08 01:13:21
MCS+tac promoter+Spacer+RBS+mCherry
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K784011_sequence (Version 1) |
Description
This is a construct containing an [[Part:BBa_K784008|MCS followed by a tac promoter]] followed by the [[Part:BBa_K784007|sapcer+RBS from theophylline riboswitch]] fused to mCherry. The construct was created using Assembly pcr. This construct was used to characterize the relationship between the theophylline concentration and the level of translation of mCherry fused to the theophylline riboswitch.The physical DNA of this part has been submitted in pSB1C3 to the registry. However, it hasn't been clones using the biobrick prefix and suffix. It has been inserted using the EcoRI and PstI restriction sites, destroying the biobrick prefix and suffix.
Notes
No design considerations.Source
Assembly pcr product of [[Part:BBa_K784008|MCS+tac promoter]] + [[Part:BBa_K784005|theophylline riboswitch]] with [[Part:BBa_J06504|mCherry]].| Access | Instance | Definition |
| public public public | BBa_K784008 BBa_K784007 BBa_J06504 | BBa_K784008 BBa_K784007 mCherry |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K784008 BBa_K784007 BBa_J06504 | 1,61 62,107 108,821 | BBa_K784008 BBa_K784007 mCherry |