Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:28
scytonemin biosynthetic pathway, scyBAC
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K814011_sequence (Version 1) |
Description
Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyB first converts tryptophan into 3-indole pyruvic acid, which is converted into a ketoacid intermediate by ScyA. ScyC then converts the ketoacid into an indole compound which spontaneously forms scytonemin.This part is made of three basic parts and contains the scyB, scyA and scyC generators. This part could be used for scytonemin biosynthesis in E. coli
Notes
N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone each gene with flanking restriction digest sites to be used for vector cloning. Each gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS. These basic parts were then assembled into the scyBAC pathway through standard BioBrick??? assembly techniquesSource
All open reading frames contained within this part came from Nostoc punctiforme PCC 73102, accession NC_010628.1| Access | Instance | Definition |
| public public public | BBa_K814008 BBa_K814007 BBa_K814009 | BBa_K814008 BBa_K814007 BBa_K814009 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K814008 BBa_K814007 BBa_K814009 | 1,1274 1283,2463 2472,4558 | BBa_K814008 BBa_K814007 BBa_K814009 |