Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alyssa Calder
Date created: 2010-04-19 11:00:00
Date modified: 2015-05-08 01:13:54
Copper Promoter (CueR regulated) with YFP Reporter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M11512_sequence (Version 1) |
Description
Promoter sequence with recognition site for the CueR transcription regulating protein. When copper levels in the cell rise CueR activates transcription (1) with enhanced yellow fluorescent protein derived from A. victoria GFP.Notes
Composite Part of BBa_K190017 and BBa_E0032Source
For further information and characterization of the CueO promotor see CueO1) K. Yamamoto and A. Ishihama (2005) Transcriptional response of Escherichia coli to external copper, Molecular Microbiology, 56(1), 215???227.
YFP
Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
| Access | Instance | Definition |
| public public public public public | BBa_K190017 BBa_B0034 BBa_E0032 BBa_B0010 BBa_B0012 | BBa_K190017 BBa_B0034 YFP BBa_B0010 BBa_B0012 |