View

BBa_M1531

BBa_M1531 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_M1531

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sarah Allred

Date created: 2012-04-17 11:00:00

Date modified: 2015-05-08 01:13:56

Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

Back

Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_M1531_sequence (Version 1)

Description

This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL1. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.

Notes

See subparts

Source

See subparts

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_K208010 BBa_K208006 BBa_B0034 BBa_M1524 BBa_M1528 BBa_B0034 BBa_K208006 BBa_K208000 BBa_M1528 BBa_B0015	BBa_K208010 BBa_K208006 BBa_B0034 BBa_M1524 BBa_M1528 BBa_B0034 BBa_K208006 BBa_K208000 BBa_M1528 BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K208010 BBa_K208006 BBa_B0034 BBa_M1524 BBa_M1528 BBa_B0034 BBa_K208006 BBa_K208000 BBa_M1528 BBa_B0015	1,220 229,414 423,434 441,947 956,4564 4573,4584 4593,4778 4785,5504 5513,9121 9130,9258	BBa_K208010 BBa_K208006 BBa_B0034 BBa_M1524 BBa_M1528 BBa_B0034 BBa_K208006 BBa_K208000 BBa_M1528 BBa_B0015