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BBa_M30009

BBa_M30009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M30009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: natalie kuldell

Date created: 2005-12-04 12:00:00

Date modified: 2015-05-08 01:13:59

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Details

Types	DnaRegion
Roles	Measurement engineered_region
Sequences	BBa_M30009_sequence (Version 1)

Description

Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of lambda cI gene. The cI protein represses expression of mRFP (M30003). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced.

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0082 BBa_B0034 BBa_C0051 BBa_B0010 BBa_B0012 BBa_R0051 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012	OmpR BBa_B0034 cI lam BBa_B0010 BBa_B0012 cI lam BBa_B0034 mRFP1 BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0082 BBa_B0034 BBa_C0051 BBa_B0010 BBa_B0012 BBa_R0051 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012	1,108 117,128 135,884 918,997 1006,1046 1055,1103 1112,1123 1130,1810 1844,1923 1932,1972	OmpR BBa_B0034 cI lam BBa_B0010 BBa_B0012 cI lam BBa_B0034 mRFP1 BBa_B0010 BBa_B0012