BBa_M30012

BBa_M30012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M30012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: natalie kuldell

Date created: 2005-12-14 12:00:00

Date modified: 2015-05-08 01:13:59

OmpR controlled mRFP

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_M30012_sequence (Version 1)

Description

Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of mRFP (I13507). Constructed with standard assembly method except R0082 was isolated using VF and VR-directed PCR. Consequently, there may be silent mutations that were inadvertently introduced. An alternative clone is M30011. Cells bearing this construct are pale pink.

Components

Access	Instance	Definition
public	BBa_R0082	OmpR
public	BBa_B0034	BBa_B0034
public	BBa_E1010	mRFP1
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0082	1,108	OmpR
BBa_B0034	117,128	BBa_B0034
BBa_E1010	135,815	mRFP1
BBa_B0010	849,928	BBa_B0010
BBa_B0012	937,977	BBa_B0012

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M30012/1

Member of these Collections

• //classic/measurement/uncategorized
• iGEM Parts Registry