Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Everett Frost
Date created: 2012-06-02 11:00:00
Date modified: 2015-05-08 01:14:03
Bicistronic Lig/MnP -Frost/Thayer
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_M36196_sequence (Version 1) |
Description
This is the entire bicistronic system that we used to create our genetic design. Ligninase is a protein used for lignin degradation by microbes and fungi. Manganese peroxidase is a necessary coenzyme for ligninase activity.Notes
This design is codon optimized for expression in escherichia coli. We had some difficulty with synthesis of the repeats in the linker sequence and repeats in the BBa_B0034 terminator.Source
http://partsregistry.org/Part:BBa_M36188, http://partsregistry.org/Part:BBa_M36192, http://partsregistry.org/Part:BBa_B0034, http://partsregistry.org/Part:BBa_M36187, http://partsregistry.org/Part:BBa_B1002| Access | Instance | Definition |
| public public public public public | BBa_M36188 BBa_M36192 BBa_B0034 BBa_M36187 BBa_B1002 | BBa_M36188 BBa_M36192 BBa_B0034 BBa_M36187 BBa_B1002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36188 BBa_M36192 BBa_B0034 BBa_M36187 BBa_B1002 | 1,1113 1122,1227 1236,1247 1254,2327 2336,2369 | BBa_M36188 BBa_M36192 BBa_B0034 BBa_M36187 BBa_B1002 |