BBa_J15506
BBa_J15506 Version 1 (Component)
spac promoter with lacI

spac+efe
BBa_K1065203 Version 1 (Component)
Efe+Bba_B0015 in pSpac (BBa_K823026)

PatgB4HT
BBa_K844016 Version 1 (Component)
Spider Silk Generator - 4x "B" Silk Construct with His Tag

BBa_K1162306
BBa_K1162306 Version 1 (Component)
Antimicrobial spider silk generator with C-terminus 10x His-Tag

BBa_K844017
BBa_K844017 Version 1 (Component)
Spider Silk Generator - 10x "F" Silk Construct with His Tag

BBa_K844005
BBa_K844005 Version 1 (Component)
Spider Silk 1x 1E Subunit "U" with Met (ATG) start codon

BBa_K1377001
BBa_K1377001 Version 1 (Component)
spaA backward + sortase A + spaB + spaC

PatgF1GFP
BBa_K844015 Version 1 (Component)
lac/IPTG inducible Spider Silk 1x "F" Subunit fused to GFP

BBa_J61207
BBa_J61207 Version 1 (Component)
TrnspsnPlsmd Tn5F|rbsGenR|SpecR|P_T7|Tn5R||CmR|TnP|OriTr|R6K

BBa_K180020
BBa_K180020 Version 1 (Component)
Rock-paper-scissors - Scissors secondary plasmid [las pR, LacI generator]

BBa_K180013
BBa_K180013 Version 1 (Component)
Rock-paper-scissors - Rock secondary plasmid [rhl pR, LacI generator]

BBa_J61212
BBa_J61212 Version 1 (Component)
Tn5F|rbsGenR|FRT|R6K|CmR|FRT|P_T7|Tn5R||SpecR|OriTr|TnP

BBa_K180015
BBa_K180015 Version 1 (Component)
Paper - Primary plasmid (part 2) [tac pR, GFP and RhlI generator]

BBa_K1162307
BBa_K1162307 Version 1 (Component)
WAM-1 antimicrobial peptide fused with 8x spider silk protein, with 10x His-Tag

BBa_K180005
BBa_K180005 Version 1 (Component)
GoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]

pSBBs0K
BBa_K823026 Version 1 (Component)
pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

GG100
BBa_K2145125 Version 1 (Component)
This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer

GG98
BBa_K2145123 Version 1 (Component)
This part contains 2 fluorescent protein coding sites (RFP and GFP) with a spacer

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.