BBa_K228813BBa_K228813 Version 1 (Component)RBS(B0034)+lacI(C0012)+terminator(B0015)
BBa_K318509BBa_K318509 Version 1 (Component)lacI pL + RBS + cI LVA + TT
RBS-LacI-TBBa_K079002 Version 1 (Component)LacI protein with terminator and RBS
BBa_K546547BBa_K546547 Version 1 (Component)Constitutive (tetR repressible) LacI and RFP expression
BBa_K876005BBa_K876005 Version 1 (Component)pLac-RBS-GFP-T-pTet-LacI
BBa_K1113411BBa_K1113411 Version 1 (Component)Targeting sequence for the delivery of the LacZ gene to the Carboxysome
CP1LacPI2BBa_K418002 Version 1 (Component)IPTG inducible Lac promoter cassette
BBa_I12026BBa_I12026 Version 1 (Component)Test of BBa_R0011 (LacI regulated) using YFP
BBa_K188122BBa_K188122 Version 1 (Component)RBS+lacI repressor from E. coli (+LVA)
BBa_K1321362BBa_K1321362 Version 1 (Component)sfGFP fused to CBDcex driven by LacI
BBa_K1472613BBa_K1472613 Version 1 (Component)∆9, ∆12, ∆15 desaturases with LacI promoter
BBa_I715071BBa_I715071 Version 1 (Component)Lac promoter with Ribosomal Binding Site
BBa_K639004BBa_K639004 Version 1 (Component)rrnB P1-LacI-pLac-mCherry plausible stress sensor
BBa_K1615108BBa_K1615108 Version 1 (Component)mRFP fused to CBDclos driven by LacI promoter
BBa_K549004BBa_K549004 Version 1 (Component)LacI promotor fused with the iron dependent regulator fur
BBa_J47053BBa_J47053 Version 1 (Component)Constitutive device (medium transcription) for lacI repressor, strong RBS
BBa_K132016BBa_K132016 Version 1 (Component)luxI+KanR-LVA+LacI+PL+KanR-LVA+aiiA+terminator
BBa_K418001BBa_K418001 Version 1 (Component)From partsregistry.org IPTG inducible Lac promoter cassette
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
BBa_K584011BBa_K584011 Version 1 (Component)Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP
BBa_J40000BBa_J40000 Version 1 (Component)Quorum sensing promoter with lac I and CFP
BBa_K1222004BBa_K1222004 Version 1 (Component)pCam(T7 promoter+lac operator+CamR antisense 2+T7 terminator)
BBa_K726009BBa_K726009 Version 1 (Component)T7 driven lac operated inducer for the rhl quorum-sensing system
BBa_K079021BBa_K079021 Version 1 (Component)LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La
BBa_J22121BBa_J22121 Version 1 (Component)Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3
BBa_K1520509BBa_K1520509 Version 1 (Component)PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.