Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lau Tsz Kit
Date created: 2014-11-01 12:00:00
Date modified: 2015-05-08 01:10:39
∆
9, ∆
12, ∆
15 desaturases with LacI promoter
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1472613_sequence (Version 1) |
Description
This protein generator is composed of the delta 9, delta 12 and delta 15 desaturase genes with an RBS upstream of each coding sequence and LacI regulated promoter. Desaturases introduce cis-carbon double bonds to the carbon chain of fatty acids. ∆9 desaturase introduces a double bond to stearic acid between carbon 9 and carbon 10 to produce oleic acid. ∆12 desaturase introduces a double bond between carbon 12 and carbon 13 of oleic acid so to produce linoleic acid. In the end, ∆15 desaturase converts linoleic acid into alpha-linolenic acid by adding a double bond between carbon 15 and carbon 16 (Figure 1).Notes
The desaturase genes in plasmids were introduced into E. coli for amplification. The final construct includes iGEM provided RBS (BBa_B0034).Source
All the desaturase sequences are derived from the Synechocystis sp. but optimised for the expression in E. coli.| Access | Instance | Definition |
| public public public public public public public | BBa_R0010 BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 | LacI BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 | 1,200 209,220 227,1186 1195,1206 1213,2274 2283,2294 2301,3384 | LacI BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 |