Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

iGEM Feature Libraries
igem_feature_libraries_collection Version 1 (Unknown)
A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

ACS Synbio Generation
ACS_Synbio_Generation Version 1 (Activity)
Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project

Free Genes Feature Libraries
free_genes_feature_libraries_collection Version 1 (Unknown)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

GFP report
BBa_E0240 Version 1 (Component)
GFP generator

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

GFP genera
BBa_E0840 Version 1 (Component)
GFP generator

BBa_J04500
BBa_J04500 Version 1 (Component)
IPTG inducible promoter with RBS

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

BBa_K249006
BBa_K249006 Version 1 (Component)
Fusion EYFP with C-terminal Arginine Tag

tetA(C)f
BBa_J31007 Version 1 (Component)
tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

BBa_K1418002
BBa_K1418002 Version 1 (Component)
Chlorophyllase (TaCHL) generator with C-terminal 10x His tag

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

LuxR
BBa_I0462 Version 1 (Component)
luxR Protein Generator

BBa_K249008
BBa_K249008 Version 1 (Component)
YFP with N-terminal Arganine Tag

BBa_K1321340
BBa_K1321340 Version 1 (Component)
Double CBD (dCBD) with N-terminal linker

BBa_K331002
BBa_K331002 Version 1 (Component)
ECFP with C-terminal Arginine Tag

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

BBa_K1333005
BBa_K1333005 Version 1 (Component)
M13 bacteriophage g8p with a FLAG tag on C-terminal

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

hixC
BBa_J44000 Version 1 (Component)
hixC binding site for Salmonella typhimurium Hin recombinase

tetA(C)b
BBa_J31006 Version 1 (Component)
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]

BBa_J13002
BBa_J13002 Version 1 (Component)
TetR repressed POPS/RIPS generator

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

HIC genera
BBa_K1602020 Version 1 (Component)
Inducible generator of Humicola insolens cutinase (HIC) with N-terminal His-Tag

BBa_I13458
BBa_I13458 Version 1 (Component)
promoter C and arabinose C operon

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

BBa_K929202
BBa_K929202 Version 1 (Component)
EGF-Receptor_3rd domain with PelB leadersequence, His-tag and C-terminal Sortase motif

RFC105 CC
BBa_K1362419 Version 1 (Component)
C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC

BBa_K1333002
BBa_K1333002 Version 1 (Component)
M13 bacteriophage g3p with start codon of "ATG" and a FLAG tag on C-terminal

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_K1162009
BBa_K1162009 Version 1 (Component)
10x-Histidine (10x-His) Tag with Met (ATG) For N-terminal purification

His6
BBa_K1362431 Version 1 (Component)
N-terminal His6 (C-Intein)

BBa_K1162015
BBa_K1162015 Version 1 (Component)
GFP with C-terminal 10x His-Tag

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

BBa_K081014
BBa_K081014 Version 1 (Component)
RFP protein generator - PoPS->RFP

PEST
BBa_J176018 Version 1 (Component)
PEST

BBa_K331001
BBa_K331001 Version 1 (Component)
ECFP with N-terminal Arginine Tag

BBa_J64101
BBa_J64101 Version 1 (Component)
ColE1 biobrick plasmid with backward terminator