HYG
Hygromicin_Resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A strain H99O

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Restriction_sites
Restriction_sites Version 1 (Component)
SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

BBa_K1150016
BBa_K1150016 Version 1 (Component)
HA tag

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

BBa_K1329001
BBa_K1329001 Version 1 (Component)
Hag-KpnI

NLS
BBa_J176008 Version 1 (Component)
NLS

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

PEST
BBa_J176018 Version 1 (Component)
PEST

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

KpnI
BBa_I11071 Version 1 (Component)
KpnI restriction enzyme site with extra flanking nucleotides

BBa_I11101
BBa_I11101 Version 1 (Component)
RBS with KpnI

BBa_K1791008
BBa_K1791008 Version 1 (Component)
KpnI cut site

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

BBa_K416003
BBa_K416003 Version 1 (Component)
Yeast Secretion Tag

RBS-GFP-Te
BBa_I763020 Version 1 (Component)
RBS-GFP (+LVA Tag)-Terminators

BBa_J70042
BBa_J70042 Version 1 (Component)
6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)

polyA
BBa_J176009 Version 1 (Component)
polyA2

BBa_J70044
BBa_J70044 Version 1 (Component)
6his part for use with BioScaffold parts (G)GATCC(glycine serine)-6his-TAA

His6
BBa_K1362431 Version 1 (Component)
N-terminal His6 (C-Intein)

BBa_J04051
BBa_J04051 Version 1 (Component)
RFP with tag

BBa_K208003
BBa_K208003 Version 1 (Component)
OmpA Signal Peptide - Silver Fusion Compatible

BBa_J70048
BBa_J70048 Version 1 (Component)
6his part for use with BioScaffold parts ATG-GGATCC (glycine serine)-6 His-TAA

ssrA(DAS+4
BBa_K1075020 Version 1 (Component)
E. coli ssrA(DAS+4)

BBa_K1149022
BBa_K1149022 Version 1 (Component)
pelB (E.coli optimized)

HisTag
BBa_J18909 Version 1 (Component)
His tag (6xHis; E. coli - optimized)

BBa_K208005
BBa_K208005 Version 1 (Component)
TorA Signal Peptide - Silver Fusion Compatible

LAA
BBa_M0050 Version 1 (Component)
AANDENYALAA. (Very fast) SsrA degradation tag.

BBa_M0051
BBa_M0051 Version 1 (Component)
AANDENYNYADAS. (Fast) SsrA degradation tag.

BBa_K496003
BBa_K496003 Version 1 (Component)
RBS + SlrP secretion tag, salmonella T3 sec. sys.

BBa_K249008
BBa_K249008 Version 1 (Component)
YFP with N-terminal Arganine Tag

BBa_K1188002
BBa_K1188002 Version 1 (Component)
RTX - Calcium Binding Protein

polyA
BBa_J176042 Version 1 (Component)
polyA

HA tag
BBa_T2011 Version 1 (Component)
hemagglutinin (HA) tag special internal domain (YPYDVPDYA)

HAT
BBa_M0033 Version 1 (Component)
HAT affinity tag

HA tag
BBa_T2010 Version 1 (Component)
hemagglutinin (HA) tag head domain (YPYDVPDYA)

BBa_K748002
BBa_K748002 Version 1 (Component)
Truncated lysostaphin coding sequence. Lysostaphin has has a specific lytic action against S.aureus.

BBa_K208002
BBa_K208002 Version 1 (Component)
Gene III Signal Peptide - Silver Fusion Compatible

BBa_J54033
BBa_J54033 Version 1 (Component)
PrefixII_SalI,BamHI

HA tag
BBa_T2009 Version 1 (Component)
hemagglutinin (HA) tag tail domain (YPYDVPDYA)

BBa_I746351
BBa_I746351 Version 1 (Component)
pag activator from PSP3 phage