Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
VRccdBBBa_K176058 Version 1 (Component)Reverse sequencing primer binds to ccdB 5prime terminal (VRccdB)
BBa_B0025BBa_B0025 Version 1 (Component)double terminator (B0015), reversed
PBS_F24PBS_F24 Version 1 (Component) BBa_J44001BBa_J44001 Version 1 (Component)Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
pSC101BBa_I50041 Version 1 (Component)pSC101 origin of replication, reverse
ccdBBBa_K145151 Version 1 (Component)ccdB coding region
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
VF2BBa_G00100 Version 1 (Component)Forward primer for sequencing/amplifying BioBrick parts (VF2)
ccdBBBa_P1010 Version 1 (Component)ccdB cell death gene
VFtetRBBa_K176057 Version 1 (Component)Forward sequencing primer binds to tetR 3prime terminal (VFtetR)
mRFPrBBa_J31008 Version 1 (Component)RFP reverse
GFP-rBBa_G00201 Version 1 (Component)Reverse primer for sequencing composite parts; binds to GFP (BBa_E0040)
M13 -47BBa_K176059 Version 1 (Component)M13 -47 general primer as a reverse primer binds to 5prime terminal of lacZ
BBa_K176063BBa_K176063 Version 1 (Component)PoPS->RBS(weak)+lacZalpha-ccdB+T
BBa_K176062BBa_K176062 Version 1 (Component)PoPS->RBS(weak)+ccdB+T
VRBBa_G00102 Version 1 (Component)Reverse BioBrick primer annealing site (VR binding site)
BBa_K176088BBa_K176088 Version 1 (Component)Reverse sequencing primer binds to the middle of luxR (VRluxR)
lacZa-ccdBBBa_K176003 Version 1 (Component)lacZalpha-ccdB coding sequence
ccdBBBa_P1016 Version 1 (Component)ccdB cassette without ccdA-
CFP/YFP-fBBa_G00200 Version 1 (Component)Forward primer for sequencing composite parts; binds to CFP/YFP (BBa_E0020/22, BBa_E0030/32)
BBa_B0024BBa_B0024 Version 1 (Component)double terminator (B0012-B0011), reversed
BBa_K1321340BBa_K1321340 Version 1 (Component)Double CBD (dCBD) with N-terminal linker
BBa_K187034BBa_K187034 Version 1 (Component)pAB/BA reverse primer for sequencing
VRBBa_G00101 Version 1 (Component)Reverse primer for sequencing/amplifying BioBrick parts (VR)
BBa_G00401BBa_G00401 Version 1 (Component)Reverse Primer to C0040
BBa_G00121BBa_G00121 Version 1 (Component)Reverse Primer to C0012
BBa_J44002BBa_J44002 Version 1 (Component)pBAD reverse
Prefix-RBBa_G1001 Version 1 (Component)Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)
BBa_G00531BBa_G00531 Version 1 (Component)Reverse Primer to C0053
BBa_G00601BBa_G00601 Version 1 (Component)Reverse Primer to C0060
BBa_G00521BBa_G00521 Version 1 (Component)Reverse Primer to C0052
BBa_G00511BBa_G00511 Version 1 (Component)Reverse Primer to C0051
BBa_G00501BBa_G00501 Version 1 (Component)Reverse Primer to C0050
BBa_K228009BBa_K228009 Version 1 (Component)AraC protein(reversed sequence) and Pbad promoter