GFPGFP Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A H99S
HYGHygromicin_Resistance Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A strain H99O
mCherrymCherry Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A H99S strain
amdSamdS Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A strain H99O
APP1GenBank_CP047908_Region_1244167_1244926 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
ACT1GenBank_CP003820_Region_1242235_1243067 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
YFPBBa_E0032 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
Truncated Sequence PullTruncated Version 1 (Activity)Used to indicate that a sequence was truncated after pulling from the source and before submission
ECFPBBa_E0022 Version 1 (Component)enhanced cyan fluorescent protein derived from A. victoria GFP
Restriction_sitesRestriction_sites Version 1 (Component)SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI
AdapterBBa_I732007 Version 1 (Component)BglII + BamHI
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
BBa_J54103BBa_J54103 Version 1 (Component)Promoterless-GFP(A)
EYFPBBa_E0034 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
BBa_K209402BBa_K209402 Version 1 (Component)AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]
BBa_I766217BBa_I766217 Version 1 (Component)Stop-D-BamHI-Adh Terminator Adaptor
lacZ aBBa_E0033 Version 1 (Component)LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
phaC1-A-B1BBa_K934001 Version 1 (Component)phaC1-A-B1 [P(3HB) synthesis]
BBa_J54033BBa_J54033 Version 1 (Component)PrefixII_SalI,BamHI
GC Optimised Sequence PullGC_Optimised Version 1 (Activity)Used to indicate that a sequence was GC optimised after pulling from the source and before submission
BamHIBBa_K150008 Version 1 (Component)BamHI restriction enzyme site
BBa_I716210BBa_I716210 Version 1 (Component)BamHI endonuclease without start codon
BamHIBBa_I11081 Version 1 (Component)BamHI restriction site with extra flanking nucleotides
BBa_J61016BBa_J61016 Version 1 (Component)Biobrick converter for BamHI-part-EcoRI
BBa_K1638016BBa_K1638016 Version 1 (Component)8 aa linker without BamHI restriction site and TEV recognition site
BBa_K209399BBa_K209399 Version 1 (Component)AarI A-D acceptor vector
A scarBBa_K106015 Version 1 (Component)AarI "A" site scar sequence
mamHIBBa_K590000 Version 1 (Component)mamHI, from <i>Magnetospirillum magneticum</i>
BBa_I716462BBa_I716462 Version 1 (Component){AraC-Pbad}{rbs1-A}{ATG}{BamHI no ATG}
BBa_J22106BBa_J22106 Version 1 (Component)rec A (SOS) Promoter