BBa_K737039
BBa_K737039 Version 1 (Component)
galK::GFP generator ligated to sRNA device controlled by aTc

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K113007
BBa_K113007 Version 1 (Component)
pBad/araC->T7 polymerase

BBa_K113009
BBa_K113009 Version 1 (Component)
pBad/araC

BBa_K113019
BBa_K113019 Version 1 (Component)
pBad/araC->T7 polymerase->GFP

BBa_K142046
BBa_K142046 Version 1 (Component)
tet-controlled LacI generator

BBa_K142016
BBa_K142016 Version 1 (Component)
tet-controlled lacI IS mutant (R197A) generator

BBa_K142017
BBa_K142017 Version 1 (Component)
tet-controlled lacI IS mutant (R197F) generator

BBa_K142018
BBa_K142018 Version 1 (Component)
tet-controlled lacI IS mutant (T276A) generator

BBa_K142019
BBa_K142019 Version 1 (Component)
tet-controlled lacI IS mutant (T276F) generator

BBa_K142020
BBa_K142020 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276A) generator

BBa_K142021
BBa_K142021 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276F) generator

BBa_K136066
BBa_K136066 Version 1 (Component)
araC/pBad - EnvZ*

BBa_K142022
BBa_K142022 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276A) generator

BBa_K142023
BBa_K142023 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276F) generator

BBa_K142047
BBa_K142047 Version 1 (Component)
tet-controlled LacI generator with constitutive TetR expression cassette

BBa_K142041
BBa_K142041 Version 1 (Component)
arabinose controlled RMF generator

BBa_K142073
BBa_K142073 Version 1 (Component)
lac-controlled GFP generator with constitutive lacI expression cassette

BBa_K142074
BBa_K142074 Version 1 (Component)
lac-controlled GFP-LVA generator with constitutive lacI expression cassette

BBa_K142075
BBa_K142075 Version 1 (Component)
lac-controlled CFP generator with constitutive lacI expression cassette

BBa_K142076
BBa_K142076 Version 1 (Component)
lac-controlled RFP generator with constitutive lacI expression cassette

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

xis/int
BBa_K112132 Version 1 (Component)
Xis Int controlled by temperature sensitive

BBa_K112147
BBa_K112147 Version 1 (Component)
lambda lysis device controlled by magnesium promoter

BBa_K112146
BBa_K112146 Version 1 (Component)
lambda lysis device controlled by PmgtCB magnesium promoter

BBa_K206030
BBa_K206030 Version 1 (Component)
Lock 1

BBa_K290000
BBa_K290000 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl

BBa_K290001
BBa_K290001 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl

BBa_K290002
BBa_K290002 Version 1 (Component)
constitutive LuxR, with bicistronic RhlI - EYFP controlled by lux pL

BBa_K290003
BBa_K290003 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - ECFP controlled by pRhl

BBa_K177025
BBa_K177025 Version 1 (Component)
GFP under araC promotor

AraC O2
BBa_R0081 Version 1 (Component)
Inhibitor (AraC loop attachment with O2 site)

BBa_J23031
BBa_J23031 Version 1 (Component)
[lock3c]

BBa_K175013
BBa_K175013 Version 1 (Component)
PTet+Lock3c

BBa_K175022
BBa_K175022 Version 1 (Component)
PTet+Lock3c+GFP+TT

BBa_K175023
BBa_K175023 Version 1 (Component)
PTet+Lock3c+cI+TT

BBa_K175026
BBa_K175026 Version 1 (Component)
PLacI+Key3c+TT+PTet+Lock3c+GFP+TT

BBa_K259005
BBa_K259005 Version 1 (Component)
AraC Rheostat Promoter

BBa_K249030
BBa_K249030 Version 1 (Component)
rpsA TIR controlled GFP

BBa_K228008
BBa_K228008 Version 1 (Component)
AraC regulatory protein

BBa_K228009
BBa_K228009 Version 1 (Component)
AraC protein(reversed sequence) and Pbad promoter

BBa_K228823
BBa_K228823 Version 1 (Component)
AND GATE lacP+SupD+AraC+RBS(J44001)+T7ptag

BBa_K190037
BBa_K190037 Version 1 (Component)
GVP with pBad/araC promoter

BBa_K228005
BBa_K228005 Version 1 (Component)
AraC protein and Pbad promoter

BBa_K235010
BBa_K235010 Version 1 (Component)
[K145303] (ribokey-controlled GFP generator)

BBa_K235011
BBa_K235011 Version 1 (Component)
[K235009][K235005] (ribokey-controlled mCherry generator)

BBa_K235013
BBa_K235013 Version 1 (Component)
[K145303][K235000] (ribokey-mediated pLac-controlled GFP reporter)

BBa_K235018
BBa_K235018 Version 1 (Component)
[K235015][K235005] pLambda-controlled mCherry generator