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BBa_K235013

BBa_K235013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K235013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kyliah Clarkson

Date created: 2009-09-02 11:00:00

Date modified: 2015-05-08 01:11:35

[K145303][K235000] (ribokey-mediated pLac-controlled GFP reporter)

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Details

Types	DnaRegion
Roles	engineered_region Reporter
Sequences	BBa_K235013_sequence (Version 1)

Description

This composite part will constitutively produce ribolocked GFP mRNA, and will produce the ribokey needed for transcription only when induced by lactose or an analogue like IPTG. This ought to reduce signal noise due to transient pLac activity.

Notes

Constructed using three-antibiotic assembly.

Source

K145303+K235000

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_J23109 BBa_J23032 BBa_E0040 BBa_B0010 BBa_B0012 BBa_R0010 BBa_J23008 BBa_J23009 BBa_B0010 BBa_B0012	BBa_J23109 BBa_J23032 GFP BBa_B0010 BBa_B0012 LacI BBa_J23008 BBa_J23009 BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23109 BBa_J23032 BBa_E0040 BBa_B0010 BBa_B0012 BBa_R0010 BBa_J23008 BBa_J23009 BBa_B0010 BBa_B0012	1,35 44,86 93,812 821,900 909,949 958,1157 1166,1259 1268,1364 1373,1452 1461,1501	BBa_J23109 BBa_J23032 GFP BBa_B0010 BBa_B0012 LacI BBa_J23008 BBa_J23009 BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K235013/1