BBa_S04522BBa_S04522 Version 1 (Component)LuxI with RFP reporter
BBa_K1960001BBa_K1960001 Version 1 (Component)Reporter controlled by sensing device
BBa_K594011BBa_K594011 Version 1 (Component)A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.
BBa_K594014BBa_K594014 Version 1 (Component)A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.
PL GFPBBa_K193000 Version 1 (Component)GFP reporter regulated by CI.
BBa_K1845000BBa_K1845000 Version 1 (Component)RFP Reporter/Screening plasmid intermediate
BBa_I20100BBa_I20100 Version 1 (Component)RBS Scaffold with GFP reporter
BBa_I23000BBa_I23000 Version 1 (Component)RBS Scaffold with mCherry reporter
BBa_K1893026BBa_K1893026 Version 1 (Component)J23119-ts-sfGFP -Gp2(star reporter)
BBa_I719022BBa_I719022 Version 1 (Component)cI promoter with GFP reporter
BBa_J92001BBa_J92001 Version 1 (Component)Lead Remover and Reporter Device
BBa_J133001BBa_J133001 Version 1 (Component)RFP Reporter Cassette B0034.E1010.B0015
BBa_K1676100BBa_K1676100 Version 1 (Component)GFP Reporter with pLac as promoter
Pr+RFPBBa_S03473 Version 1 (Component)Lambda-system Pr promoter with RFP reporter
BBa_I732753BBa_I732753 Version 1 (Component)P_U073O22+D001O47 + Double Reporters
BBa_J69591BBa_J69591 Version 1 (Component)GFP reporter device - using Standard Promoter (1RPU)
BBa_K775012BBa_K775012 Version 1 (Component)diacetyl chimeric GPCR with Fus1-EGFP reporter (2)
BBa_I732747BBa_I732747 Version 1 (Component)P_U073O27+D062NUL + Double Reporters (lacZa+RFP)
BBa_K779121BBa_K779121 Version 1 (Component)Short DNA reporter top strand (with RQ quencher) MammoBlock
BBa_K779116BBa_K779116 Version 1 (Component)Short RNA reporter bottom strand (with ROX fluorophore) MammoBlock
BBa_K1952012BBa_K1952012 Version 1 (Component)Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter
BBa_K1606010BBa_K1606010 Version 1 (Component)Kumamax enzyme that gluten updated m-cherry reporter device
BBa_K395102BBa_K395102 Version 1 (Component)GFP reporter repressed by LuxR and 3OC6HSL (K395005:K121013)
BBa_K395103BBa_K395103 Version 1 (Component)GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)
BBa_J58014BBa_J58014 Version 1 (Component)Promoter activated by OmpR-P with the reporter GFP
BBa_K1114500BBa_K1114500 Version 1 (Component)MoClo Level 1 RFP reporter with AE fusion sites
BBa_K812130BBa_K812130 Version 1 (Component)Citrine reporter with a Kozak sequence for expression in Xenopus
Pr+CFP-BBa_S03475 Version 1 (Component)Lambda-system Pr promoter with CFP(no degradation tag) reporter
BBa_J58015BBa_J58015 Version 1 (Component)Mutated promoter activated by OmpR-P with the reporter GFP
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
CMV + GFPBBa_K1852000 Version 1 (Component)GFP + CMV Promoter used for expression in oocyte cells. This part was planned to be used as a report
BBa_K1796201BBa_K1796201 Version 1 (Component)An unloaded sgRNA that contains BbsI cutting site, with a promoter and terminator.
[OriTR]+[RBBa_K1439002 Version 1 (Component)This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.
BBa_K779120BBa_K779120 Version 1 (Component)RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488) MammoBlock
BBa_K1088052BBa_K1088052 Version 1 (Component)GFP reporter with flexible linker at N-terminus for creation of GFP fusions
BBa_I732091BBa_I732091 Version 1 (Component)Double Repoters (LacZ-alpha and GFP-AAV)
BBa_K079051BBa_K079051 Version 1 (Component)LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator
BBa_K1778005BBa_K1778005 Version 1 (Component)eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.